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Influence of Variety and Harvest Date on Sweet Potato Weevil Damage UVI AES Biotechnology & Agroforestry Thomas W. Zimmerman, Stafford M.A. Crossman, Carlos Montilla, Noel Burnett, Chinaemere Igwebuike, Kenya Emanuel Student project to identify viruses in Sweet Potato From the six growing areas, all had 1-3 viruses Yellow represent Potyvirus, Blue represent Cucumber Mosaic Virus and Red represent Zucchini Yellow Mosaic Virus Sweet Potato Varieties USDA* Francia Gonime Liberty Mojave Okinawa Ruddy Toquecita White Jewel Yellow Sunflower Caribbean Craneal PR 406 PR 8023 Pujol St Kitts Sweet James LSU* B-14 Evangeline Murasaki Virus-Free material arrived as 1-2 two test tubes and needed to be micropropagated in vitro Sweet Potato grown in vitro with multiple sucrose concentrations 0 1 2 3 4 5 6 0 5 10 15 20 25 30 35 40 45 Days Leaves 0% 3% 6% 9% 12% 0 1 2 3 4 5 6 0 5 10 15 20 25 30 35 40 45 Leaves Days 0% 0.1% 0.3% 1% 180 Days In vitro plants were grown under a 16 hr photoperiod at 25C Rooted shoots were transferred to sterilized potting mix, covered for a week to assist in acclimatizati …
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Influence of Variety and Harvest Date on Sweet Potato Weevil Damage UVI AES Biotechnology & Agroforestry Thomas W. Zimmerman, Stafford M.A. Crossman, Carlos Montilla, Noel Burnett, Chinaemere Igwebuike, Kenya Emanuel Student project to identify viruses in Sweet Potato From the six growing areas, all had 1-3 viruses Yellow represent Potyvirus, Blue represent Cucumber Mosaic Virus and Red represent Zucchini Yellow Mosaic Virus Sweet Potato Varieties USDA* Francia Gonime Liberty Mojave Okinawa Ruddy Toquecita White Jewel Yellow Sunflower Caribbean Craneal PR 406 PR 8023 Pujol St Kitts Sweet James LSU* B-14 Evangeline Murasaki Virus-Free material arrived as 1-2 two test tubes and needed to be micropropagated in vitro Sweet Potato grown in vitro with multiple sucrose concentrations 0 1 2 3 4 5 6 0 5 10 15 20 25 30 35 40 45 Days Leaves 0% 3% 6% 9% 12% 0 1 2 3 4 5 6 0 5 10 15 20 25 30 35 40 45 Leaves Days 0% 0.1% 0.3% 1% 180 Days In vitro plants were grown under a 16 hr photoperiod at 25C Rooted shoots were transferred to sterilized potting mix, covered for a week to assist in acclimatization. After two weeks, they were transferred to a greenhouse. Replicated trial 1 ft between plants 4.5 ft between rows, 9 varieties/row, 17 plants/variety/row, 3 rows/block, 3 blocks Six weevil traps placed throughout the field and monitored weekly Sweet potatoes ready for vine removal and harvest Harvesting 0 4 8 12 16 20 24 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 Weevils Week Average number of male weevils captured weekly per trap 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 100 Days 115 Days 130 Days Average Kg Marketable Tuberous Sweet Potato Roots per Plant Variety Kg Average Number of Marketable Tuberous Sweet Potato Roots per Plant Variety 0 1 2 3 4 5 6 100 Days 115 Days 130 Days R O O T S 0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Weevil small Mkt 0% 10% 20% 30% 40% 50% 60% 70% 80% 90% 100% Weevil small Mkt Comparison between 100 and 130 day for Marketable, Small and Weevil damaged Sweet Potatoes 130 Days 100 Days Taste Evaluation Refrigerated Storage At harvest soluble solids (sugars) 6-8% After 2 weeks soluble solids increases to 8-13% After 4 weeks soluble solids range 10-16% Future studies on postharvest to determine refrigeration vs room temperature storage influence on sugar content Conclusions Sweet potato viruses are present throughout St Croix 0.1-0.3% sucrose can used for extended culture life 1 year Weevils are present throughout sweet potato production Harvest at 100 days minimizes weevil damage Varieties B-14, Francia, Mojave, Pujol, PR 8023 and Toquecita produce over 1 Kg/plant in 100 days Varieties with the greatest weevil resistance at 130 days were Gonime, Liberty, St Kitts, Sweet James, Toquecita and Yellow Sunflower Refrigeration increases soluble sugar content Acknowledgements This research was supported through a grant from the VI Department of Agriculture Specialty Crops Block Grant Program and USDA-Hatch. Special thanks to my students and Biotechnology & Agoforestry field staff: James Gordon Henry Harris Raheem Smart Mike Morgan Anna Bastien-Gilbert Kalunda Cuffy Tyrone Pascal Jomanni Bernier Khalid Matthew Coauthors: Stafford M.A. Crossman Carlos Montilla Noel Burnett Kenya Emanuel Chinaemere Igwebuike